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肿瘤坏死因子α(TNFα)4折促销
1459 人阅读发布时间：2014-08-06 15:05
肿瘤坏死因子α(TNFα)重组蛋白4折促销
原价格：2685元/100ug ，现在价格：1074元。
18702156455 李先生
原价格：2685元/100ug ，现在价格：1074元。
肿瘤坏死因子α(TNFα)重组蛋白
Recombinant Tumor Necrosis Factor Alpha (TNFa)
产品编号RPA133Hu01
适用生物Homo sapiens (Human，人)
品牌商Cloud-Clone Corp. (美国)
产品应用SDS-PAGE; WB; ELISA; IP.规格100ug
分子量21.2kDa
价格 1342元
纯度> 95%
原核表达宿主E.coli
上海研卉生物科技有限公司
Recombinant Tumor Necrosis Factor Alpha (TNFa)
产品编号RPA133Hu01
适用生物Homo sapiens (Human，人)
品牌商Cloud-Clone Corp. (美国)
产品应用SDS-PAGE; WB; ELISA; IP.规格100ug
分子量21.2kDa
价格 1342元
纯度> 95%
原核表达宿主E.coli
Recombinant Tumor Necrosis Factor Alpha (TNFa)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Homo sapiens (Human) |
| Product No. | RPA133Hu01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 21.2kDa |
| Predicted isoelectric point | 7.9 |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Subcellular Location | Secreted. Cell membrane; Single-pass type II membrane protein |
| Residues | Val77~Leu233 (Accession # P01375) with two N-terminal Tags, His-tag and T7-tag |
| Formulation | Supplied as lyophilized form in 20mM Tris, 500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative. |
SEQUENCES
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- VRSS SRTPSDKPVA HVVANPQAEG QLQWLNRRAN ALLANGVELR DNQLVVPSEG LYLIYSQVLF KGQGCPSTHV LLTHTISRIA VSYQTKVNLL SAIKSPCQRE TPEGAEAKPW YEPIYLGGVF QLEKGDRLSA EINRPDYLDF AESGQVYFGI IAL
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- VRSS SRTPSDKPVA HVVANPQAEG QLQWLNRRAN ALLANGVELR DNQLVVPSEG LYLIYSQVLF KGQGCPSTHV LLTHTISRIA VSYQTKVNLL SAIKSPCQRE TPEGAEAKPW YEPIYLGGVF QLEKGDRLSA EINRPDYLDF AESGQVYFGI IAL
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
18702156455 李先生